|
KMID : 1094720220270060975
|
|
Biotechnology and Bioprocess Engineering
2022 Volume.27 No. 6 p.975 ~ p.986
|
|
|
Activation of Heme Oxygenase-1 is Involved in the Preventive Effect of Honokiol against Oxidative Damage in Human Retinal Pigment Epithelial Cells
|
|
Hong Su-Hyun
Park Cheol
Hwangbo Hyun
Bang Eun-Jin
Kim Sung-Ok
Shim Jung-Hyun
Park Shin-Hyung
Lee Hye-Sook
Leem Sun-Hee
Kim Gi-Young
Choi Yung-Hyun
|
|
|
Abstract
|
|
|
|
Previous studies have shown that honokiol, a bioactive compound originated from the Magnolia, has beneficial effects on various chronic diseases. Although our previous study has demonstrated that honokiol effectively protects C2C12 myoblasts against hydrogen peroxide (H2O2)-induced oxidative stress and cytotoxicity, the antioxidant mechanism in human retinal pigment epithelial (RPE) cells has not been reported yet. Therefore, this study investigates whether honokiol attenuate cytotoxicity of H2O2 to human RPE ARPE-19 cells. Our results showed that H2O2-induced loss of cell viability and increase of reactive oxygen species production in ARPE-19 cells were significantly alleviated by honokiol, which was associated with increased phosphorylation of nuclear factor erythroid 2-related factor 2 (Nrf2) and upregulation of heme oxygenase-1 (HO-1). In addition, honokiol markedly attenuated DNA damage and G2/M phase cell cycle arrest and improved apoptosis in H2O2-treated ARPE-19 cells. Furthermore, mitochondrial dysfunction by H2O2 was reversed by honokiol through preservation of mitochondrial membrane potential, decrease of Bax/Bcl-2 expression ratio, and inhibition of cytochrome c release into the cytoplasm. However, zinc protoporphyrin, an inhibitor of HO-1, significantly abrogated these preventive effects, suggesting a critical role of Nrf2/HO-1 in the antioxidant activity of honokiol. Taken together, these results demonstrate that activation of Nrf2/HO-1 antioxidant signaling by honokiol could rescue ARPE-19 cells from oxidative damage.
|
|
|
KEYWORD
|
|
|
honokiol, Nrf2/HO-1, reactive oxygen species, retinal pigment epithelial cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|